T细胞在胸腺里的发育过程的细胞和分子动力学一直是人们感到好奇的领域。现在,动态作图技术给这个复杂问题的解决提供了机会。特别是双光子激光扫描显微镜(two-photon laser scanning microscopy)的发展,大大促进了组织内部的淋巴细胞行为的研究。这种技术能分辨单个胸腺细胞的行为模式,能在时间和空间上研究胸腺选择事件。
这篇综述的要点:
Inherent challenges for imaging T-cell development;
Preparations for imaging some aspects of T-cell development;
Thymocyte trafficking in the thymus;
Thymocyte interactions with thymic stromal cells;
The immunological synapse during thymic selection events.
参考文献:
Real-time imaging of T-cell development.
Philippe Bousso.
Current Opinion in Immunology 2004, 16:400–405.
DOI 10.1016/j.coi.2004.05.015
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=15245732
http://dx.doi.org/10.1016/j.coi.2004.05.015