the advantage of such binding for SSB activity?

Group 1

Replication fork,

Telomeres, Histones,

Nucleotides,

Nucleosides

1 Why is "conservative site-specific recombination" said to be "conservative?"

2 Describe the differences between the DNA and RNA simply?

3 How is the DNA unwound at the replication fork? What effect does this have on the DNA

upstream of the fork, and how does the cell deal with this effect?

4 Address how cooperative binding contributes to SSB function during DNA replication. What is

the advantage of such binding for SSB activity?

Group 2

名词解释：

transposon,

replication fork,

replicator,

mating-type switching,

homologous recombination,

telomerase

问答：1. The size of Okazaki fragments differs in different organisms. What might influence this

size?

2. Identify the major steps of homologous recombination. What bacterial and eukaryotic

proteins are responsible for carrying out each of these steps?

3. What is the end replication problem? How does the telomerase solve the end replication

problem in Eukaryote cells?

4. What is the function of the two divalent metal ions in the palm domain of DNA

polymerase during replication?

Group 3

Unit1-5

1. Design an experiment which proves that DNA can carry genetic information.

2. What is the advantage of G between 2 and 5 kcal/mol? △

Unit6

1． Other than hydrogen bonding, what else contributes to the stability of the double helix? .

2． Certain chemical agents such as nitrous acid can deaminate cytosine, converting it into uracil.

How might this explain why DNA contains thymine in place of uracil?

Unit7

1. What are the "tails" of the histone proteins, and how are they distinguished from the rest of the

protein?

2. Describe the process by which the histone tail modifications that are present at a given

genomic region are maintained during DNA replication. How do the histones distribute during

replication compare to the semi-conservative replication of DNA itself?

Unit8

1. Please summarize the function of the DNA polymerase, involv ing finger, palm and thumb

domain.

2. Please briefly describe the steps of the DNA replication and function of enzymes that anticipate

the replication.

Unit9

1. Describe why the cell can use repair mechanism to deal with x-rays damage?

2. What is the role of base flipping in DNA repair? What does the repair machinery accomplish

by relying on this phenomenon?

Unit10

1. Describe the roles of RuvA and RuvB in promoting branch migration. What are the genetic

consequences of branch migration occurring during recombination?

2. How do chi sites protec t E. coli against foreign DNA? What are the steps involved in the

recognition of chi sites by the recombination machinery?

Unit11

1. Cite three biological processes that rely on conservative site-specific recombination. What

type of rearrangement is involved in each process?

2. Describe the reactions mediated by serine and tyrosine recombinases. What role do the

serine and tyrosine side chains play respectively in the correlative reactions?

Group 4

一、名词解释

1、initiator 2、replicator 3、replicon 4、replication fork 5、RecBCD pathway

6、Holliday junction

二、问答题

1、真核生物中，同源重组和交配型交换中引入双链缺口的En 分别是什么？有何异同？

2、请简述原核生物DNA 复制过程及每一步中涉及的酶及其作用。DNA 全酶分组分及其

作用？

3、What is the differences between the cut-and-paste mechanism of transposition and the

replicative mechanism of transposition ?

Group 5

1. What is “ fail-safe systems” ?

2. What is “replicator”?

3. What is cooperative binding of SSB?

4. What is the function of initiators in Prokaryotic and Eukaryotic cells ?

5. Describe the steps of DSB-recombination .

Group 6

1. Describe the steps of Holliday Model recombination .

2. Name three of the DNA Repair Systems Briefly and describe them .

3. What is the DNA helicase and its function .

4. Address how cooperative binding contributes to SSB function during DNA replication .

5. Give the explanation of histone ; heat denaturation ; replication machinery ; heat denaturation

Group 7

1. Explain those words and phrases :

Nucleosome

Sliding DNA clamp

Replication fork

Origins of replication

Telomerase

Transition

Transversion

DSB

2. what is the the importance of packing of DNA into chromosomes ?

3. what is the functional difference of the two types of topoisomerases?

4. what is the DNA Pol III holoenzyme composed of ?

Group 8

1. How RNA primers are removed when DNA replication ended?

2. How mating-type switching is initiated?

3. What is the promblems of the replication of the lagging strand, how does the replication show

it?

4. What is the function of SSB?

5. Compare with the Homologous Recombination and Site-specific Recomination

6. Describe the difference in the process between Mating-Type Switching and DSB model.

7. Which protein in Eukaryotic make the introduction of DSB?

8. What is the difference in the function of the two kinds of topoisomerases?

Group 9